Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
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Molecular Devices Photometric Filter, 570 nm, Absorbance, Replacement
AD 340 photometric filter for 570 nm absorbance measurements. This replacement optical filter isolates the 570 nm band for photometric assays, improving wavelength specificity and measurement reliability in absorbance-based detection systems.
- Center wavelength 570 nm for absorbance measurements.
- Designed for photometric wavelength selection in plate readers.
- Helps improve assay specificity and reduce spectral interference.
- Compact optical filter form factor for easy installation and replacement.
- Suitable for routine maintenance and filter replacement in absorbance detection workflows.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Molecular Devices fluorescence band-pass filter supplied with a holder, designed to provide selective optical filtering for fluorescence assays. The filter has a center wavelength of 465 nm and a nominal full width of 35 nm, enabling targeted detection of blue/green fluorophores in multi-mode microplate reader applications.
- Provides a center wavelength of 465 nm for blue/green fluorophore detection.
- 35 nm full width for controlled spectral selection and reduced cross-talk.
- Includes a holder for secure mounting in the instrument filter tray or slide slot.
- Intended for fluorescence detection in multi-mode microplate assays.
- Helps reduce background and improve signal specificity in fluorescence measurements.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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The Enhanced TRF Module expands time-resolved fluorescence performance of SpectraMax iD5 and iD5e microplate readers, enabling TRF, TR-FRET, HTRF, IMAP, and ScanLater western blot reads with improved sensitivity for lanthanide assays.
- Improved sensitivity for Europium detection down to 10 fM.
- Enables TRF, TR-FRET, and HTRF assay read modes.
- Supports ScanLater western blot TRF reads.
- Operates with configurable flash intervals for time-resolved reads.
- Compatible with exchangeable excitation and emission filter slides sold separately.
- Requires compatible data acquisition software for some TR-FRET read modes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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