Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF Human Total H2AX Detection Kit, 10,000 Assay Points
HTRF Human Total H2AX Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Ataxin 2 Mutant, Control Lysate
HTRF Human Ataxin 2 Mutant, Control Lysate
Perkin Elmer US LLC Complete Capillary Injector Add On Kit for 240 V AutoSystem
Complete Capillary Injector Add On Kit for 240 V AutoSystem
Revvity Health Sciences Inc HTRF Human XIAP / BIR2 Binding Kit, 100,000 Assay Points
HTRF Human XIAP / BIR2 Binding Kit, 100,000 Assay Points
Precision Glassblowing of Colorado TJA INJECTOR 1.0MM 12540701
Quartz Injector, 1.0mm (2 red rings), for TJA 61E, Iris & Intrepid Radial, and for TJA 61E Trace, Iris AP, & Intrepid
Revvity Health Sciences Inc HTRF Human Total TFEB Detection Kit, 50,000 Assay Points
HTRF Human Total TFEB Detection Kit, 50,000 Assay Points
Molecular Devices Absorbance Filter, 260 nm, 5.5 nm Bandwidth
The Molecular Devices 260 nm absorbance filter (5.5 nm bandwidth) is an optical bandpass accessory designed for use with compatible FilterMax series multimode microplate readers. It provides a narrow UV transmission centered at 260 nm for precise absorbance measurements of nucleic acids and other UV-absorbing analytes.
- Provides narrow bandpass transmission centered at 260 nm.
- 5.5 nm bandwidth for selective UV absorbance measurements.
- Suitable for nucleic acid quantification and other UV-absorbing samples.
- Designed for easy installation in modular filter holders.
- Improves measurement specificity by reducing out-of-band light.
Molecular Devices Fluorescence Emission Filter, 550 nm, 10 nm
Emission filter centered at 550 nm with a 10 nm full width at half maximum (FWHM), intended for selective fluorescence detection in multi-mode microplate readers that accept standard filter slides or mounts.
- Center wavelength 550 nm.
- Bandwidth 10 nm (FWHM).
- Emission bandpass design for fluorescence detection.
- Reduces spectral crosstalk and improves signal specificity.
- Suitable for use in multi-mode microplate readers and compatible filter slide systems.
Molecular Devices Fluorescence Filter, 535 nm, 25 nm, With Holder
Molecular Devices fluorescence emission filter with integrated holder provides a narrow 535 nm center wavelength and 25 nm bandwidth for selective detection of fluorophores emitting near 535 nm. It is intended for filter-based microplate readers and supports fluorescence and TR-FRET assay detection, including TAMRA-based assays.
- Center wavelength 535 nm.
- Bandwidth 25 nm.
- Includes integrated holder for installation in filter slot.
- Suitable for fluorescence and TR-FRET detection workflows.
- Designed for use in filter-slot multi-mode microplate readers.
Molecular Devices Time-Resolved Emission Filter, 520 nm, 15 nm Bandwidth
Time-resolved emission filter centered at 520 nm with a 15 nm bandwidth, supplied with a holder for installation in compatible microplate readers. Designed to support time-resolved fluorescence detection workflows including TR-FRET and IMAP assays, the filter provides narrow spectral selection and supports temporal gating to improve signal specificity.
- Center wavelength 520 nm
- Spectral bandwidth 15 nm
- Filter type time-resolved emission
- Includes holder for microplate reader installation
- Optimized for time-resolved fluorescence and TR-FRET/IMAP assays
- Improves signal-to-noise in temporal gating measurements
Molecular Devices Fluorescence Polarization Filter, 595 nm, 35 nm BW
A fluorescence polarization emission filter optimized for 595 nm detection with a 35 nm bandwidth and vertical polarization. Designed for fluorescence polarization assays, the filter improves signal selectivity and reduces background when detecting TAMRA-like red fluorophores. It is compatible with filter-based microplate readers.
- Center wavelength 595 nm for red emission detection.
- 35 nm bandwidth to balance sensitivity and selectivity.
- Vertical polarization for fluorescence polarization measurements.
- Suitable for use in filter-based microplate reader filter holders.
- Optimized for TAMRA and similar red fluorophores.
Molecular Devices Excitation Filter, 540 nm, 25 nm Bandwidth, Bandpass
Molecular Devices FilterMax fluorescence excitation bandpass filter centered at 540 nm with a 25 nm full width, designed for targeted excitation in fluorescence microplate assays. It reduces out-of-band light to improve assay signal-to-noise and installs into compatible filter slide slots for rapid configuration.
- Provides 540 nm excitation with 25 nm bandwidth.
- Bandpass design reduces background and improves signal specificity.
- Optimized for microplate-based fluorescence assays.
- Fits into compatible filter slide slots for quick installation.
Molecular Devices Fluorescence Polarization Filter, Polarized Horizontal, 400-750 nm
The fluorescence polarization filter is an accessory optical filter designed for polarized fluorescence measurements on compatible microplate readers. It provides a horizontal polarization orientation and covers broad visible wavelengths; the filter is supplied with a holder for installation in the instrument's filter cartridge or slide system.
- Polarized horizontal orientation for fluorescence polarization assays.
- Covers 400 nm-750 nm wavelength range.
- Supplied with a holder for installation in the filter cartridge or slide.
- Designed for use in compatible microplate readers to enable polarized fluorescence detection.